Standard Operating Procedures

Ammonia Solution

This chemical standard operating procedure (SOP) is for your safety. Read, understand, and follow this document. If this is a paper copy, you should check for the most recent version on the IsoLab website. This document was created from a template modeled after the University of Washington Environmental Health and Safety Laboratory Safety Manual Standard Operating Procedures Components.

Is Ammonia Solution a particularly hazardous substance? YES

What is a particularly hazardous substance? The University of Washington Environmental Health and Safety defines it here.

The SDS for Ammonia Solution can be found here.

1. Process Ammonia solution (also known as aqueous ammonia or ammonium hydroxide) is used in the Chromium Reduction protocol to capture H2S.
2. Hazards Ammonia solution is also known as aqueous ammonia or ammonium hydroxide. It is harmful if swallowed, causes severe skin burns and eye damage, may cause respiratory irritation, and is very toxic to aquatic life. Refer to the SDS for more information.
3. Personal Protective Equipment While working with ammonia solution in a fume hood you should be wearing nitrile gloves, a lab coats, and eye protection. Do not work with ammonia solution outside of the fume hood.
4. Environmental / Ventilation Controls Ammonia solution is to be used in one of the fume hoods in the 302 / 303 suite. Ensure the fume hood has appropriate flow prior to working with ammonia solution. Do not work with ammonia solution unless the fume hood is functioning properly.
5. Special Handling Procedures & Storage Requirements Ammonia solution is stored in the corrosives cabinet under the west fume hood in 303B. Make sure you have your laboratory coat, eye protection, and gloves on before handling the bottle. Consider making a working solution container so that the stock bottle can remain tightly closed and in the corrosives cabinet.
6. Spill and Accident Procedures

Inside Fume Hood Spill - The fume hood should protect you from fumes. Contain the solution with absorbent materials from the spill kit and place in a suitable waste container.

Outside Fume Hood Spill - FUMES - Everyone should evacuate. If you intend to clean up the spill yourself, don a respirator with an ammonia cartridge and non-vented protective goggles before attempting clean-up.

7. Waste Disposal All ammonia solution waste is contained in an appropriate labeled tightly sealable plastic, wide-mouth, container. Do not open the waste container unless it is contained in a fume hood. Contact EH&S for waste removal when the container is sufficiently full or aged.
8. Special Precautions for Animal Use (if applicable) N/A
Particularly hazardous substance involved? YES, see 9-11
9. Approval Required

You must have taken Managing Laboratory Chemicals or similar UW EH&S condoned course before using ammonia solution. You must have read and understood the SDS for Ammonia solution as well as this SOP. You also must review your process with previously trained laboratory personnel.

10. Decontamination Any glassware that has been used with ammonia solution should be rinsed such that the first rinse goes into the above mentioned waste container. Subsequent rinse or washing of glassware can be done outside the fume hood in the sink as per normal lab glassware cleaning procedures.
11. Designated Area Ammonia solution may only be used in a fume hood. Preferably the fume hood directly above its storage location.


I have read and understood this Chemical SOP:

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Last updated: 2020-10-13 11:07:32