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Standard Operating Procedures

Cleaning Laboratory Glassware

Introduction

This document describes how to clean all sample preparation glass and plastic ware.

Safety

You will be using a strong soap (micro-90) and very high temperatures. You should wear gloves to protect your hands from the soap. You should also consider wearing a lab coat to protect your clothing. When using the muffle oven, wear protective gloves if the glass is still warm. You are expected to have read the muffle furnace SOP.

Terse Procedure

  1. Wash dishes with soap and water
  2. All debris, oils, labeling tape, sharpie labels, and sharp objects should be gone
  3. Rinse with tap water
  4. Rinse with DI water
  5. Dry in hood
  6. Wrap or top with foil as appropriate
  7. Bake in muffle as appropriate
  8. Return clean dishes to appropriate location

Cleaning Glassware

  1. Don
    glovesNitrile gloves are located on the north wall shelves in 303B closest to the windows and microbalances.
  2. Remove any tape or foreign matter from the glassware and rinse if needed.
  3. Place glassware in a tub of
    Micro-90micro 90 soapMicro-90 is a concentrated soap and is located under the sink of room 303B closest to the office (the northernmost sink). If the 4 L plastic container is not there, look for a new container on the top central shelf above all other reagents in 303B on the north wall (storage shelf #6).
    soapy water. Difficult-to-clean glassware may need overnight soaking and if soaking, ensure the entire piece is covered with soapy water. Choosing to soak or not will depend on how much time you have to spend doing dishes and how quickly you need the dishes clean. If soaking, ensure the entire piece is covered with soapy water. If not soaking, or when finished soaking, proceed to next steps.
  4. Scrub as needed the inside and outside of glassware with brush or sponge. 
  5. Rinse thoroughly with tap water.
  6. Rinse well with deionized (DI) water.
  7. Place glassware on drying paper in the gray bin located in the fume hood, and allow to completely dry.
  8. Once dry, cover with foil and bake in muffle furnace at 500 °C. Make sure to read the muffle furnace SOP and fill out the sign-in sheet. Do not bake laser vials with inserts.
  9. Once dry or baked in muffle furnace, return glassware to the appropriate storage location.

Cleaning water laser vials

The laser vials with 300 µL inserts can not be cleaned in the normal way. The air space between the insert and the vial wall keeps the vial afloat. If the insert appears to have salts or otherwise look dirty, use these steps to attempt a cleaning.

  1. Start by placing dirty vials into a blue tray as if the vials had sample water in them.
  2. Pipette DI water into each vial, making sure air bubbles are removed from bottom of vial insert.
  3. Cover with vials with glass plate and allow them to soak like this overnight.
  4. Fling DI water into sink.
  5. Attach glass pipette flexible hose piece to DI water spigot.
  6. Turn DI water on only slightly and rinse each vial insert thoroughly.
  7. Allow vials and tray to dry in drying oven overnight.
  8. Inspect inserts and ensure they are clean. If not, repeat or discard into broken glass.

Cleaning Bacterial Denitrifier Sample Vials

Crimped-cap removal tool and gloves
Crimped-cap removal tool and gloves. Both are stored in the open drawer that is pictured.

The bacterial denitrifier method sample vials require a special cleaning method because of the oil used to lubricate the septum on the autosampler.

  1. Wear
    thick protective glovesYou can try using leather gloves under nitrile gloves. Leather gloves are located in the garage (302C) and nitrile gloves are on the north wall shelves near the window and microbalances. You can also try using the thicker gloves located in the HF lab (302B) located on the north wall shelves. Do not use the autoclave gloves that are stored in the same drawer as the cap removal tool.
    that will withstand broken glass abrasion. Remove the crimped aluminum ring using the
    removal toolThe removal tool is located in the denitrifier supplies drawer on the east wall under the laminar flow hood / box.crimp cap removal tool
    . Empty vial contents into "high-pH waste" container. If the glass vial breaks, place glass pieces in broken glass container after emptying liquid into high-pH waste container.
  2. Cover the bottom of a red dish tub with 100% Micro-90 and cover vials with hot water.
  3. Completely submerge empty vials, make sure most air is out of each vial and soak for 10-20 minutes. 
  4. Transfer, and completely submerge the vials into the second red dish tub of soapy water.
  5. Clean the inside/outside of the glassware thoroughly with a brush/sponge. 
  6. Rinse vials thoroughly and feel with bare hands for grease. If grease is still present, rewash the vials. If they feel clean, continue with step 4 of the Cleaning Glassware procedure. When you are at the muffle furnace stage, wrap 20-30 vials with foil into a brick shape and then place them in the furnace.

Cleaning Plasticware

  1. Follow glassware steps 1-6.
  2. DO NOT PUT PLASTIC IN THE MUFFLE OVEN.
  3. Once dried in the fume hood overnight, return plasticware to the appropriate storage location.

Broken Glass or Plastic

If you break any glassware or plasticware during this procedure, make certain all broken pieces are put into the broken glass box located in room 303B under the lab bench near the northernmost sink.

Last updated: 2020-10-18 14:27:48