Acetic Anhydride

This chemical standard operating procedure (SOP) is for your safety. Read, understand, and follow this document. If this is a paper copy, you should check for the most recent version on the IsoLab website. This document was created from a template modeled after the University of Washington Environmental Health and Safety Laboratory Safety Manual Standard Operating Procedures Components.

Is Acetic anhydride a particularly hazardous substance? YES

What is a particularly hazardous substance? The University of Washington Environmental Health and Safety defines it here.

The SDS for acetic anhydride can be found here.

1. Process Acetic anhydride is used to make the resin for selectively extracting selenium from solutions for later isotopic analyses.
2. Hazards Acetic anhydride is a flammable liquid and vapor, harmful if swallowed, harmful if inhaled, and causes severe skin burns and eye damage. Refer to the SDS for more information.
3. Personal Protective Equipment While working with acetic anhydride in a fume hood you should be wearing nitrile gloves, a lab coats, and eye protection. Do not work with acetic anhydride outside of the fume hood.
4. Environmental / Ventilation Controls Acetic anhydride is to be used in one of the fume hoods in the 302 / 303 suite. Ensure the fume hood has appropriate flow prior to working with acetic anhydride. Do not work with acetic anhydride unless the fume hood is functioning properly.
5. Special Handling Procedures & Storage Requirements Acetic anhydride is stored in the fume hood flammables cabinets of 302A and 302B. Make sure you have your laboratory coat, eye protection, and gloves on before handling the bottle.
6. Spill and Accident Procedures

ELIMINATE all ignition sources (no smoking, flares, sparks or flames in immediate area). Keep unauthorized personnel away. Keep upwind. Use personal protective equipment. Do not touch damaged containers or spilled material unless wearing appropriate protective clothing. Ventilate closed spaces before entering them. See Section 8 of the MSDS for Personal Protective Equipment.

Eliminate all ignition sources if safe to do so. Take precautionary measures against static discharges. Stop leak if possible without any risk. Use only non-sparking tools. Absorb spill with vermiculite or other inert material, then place in a container for chemical waste. Clean surface thoroughly to remove residual contamination. Dike far ahead of larger spill for later recovery and disposal.

Prevent entry into waterways, sewer, basements or confined areas. Inform authorities if large amounts are involved.

Do not contaminate water sources or sewer. Prevent further leakage or spillage if safe to do so.

7. Waste Disposal

All acetic anhydride waste is contained in an appropriate labeled tightly sealable plastic, wide-mouth, container. Do not open the waste container unless it is contained in a fume hood. Contact EH&S for waste removal when the container is sufficiently full or aged.

An empty acetic anhydride container should be treated as acetic anydride.

8. Special Precautions for Animal Use (if applicable) N/A
Particularly hazardous substance involved? YES, see 9-11
9. Approval Required

You must have taken Managing Laboratory Chemicals or similar UW EH&S condoned course before using acetic anhydride. You must have read and understood the SDS for Acetic anhydride as well as this SOP. You also must review your process with previously trained laboratory personnel.

10. Decontamination Any glassware that has been used with acetic anhydride should be rinsed such that the first rinse goes into the above mentioned waste container. Subsequent rinse or washing of glassware can be done outside the fume hood in the sink as per normal lab glassware cleaning procedures.
11. Designated Area Acetic anhydride may only be used in a fume hood. Preferably the fume hood directly above its storage location.


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Last updated: 2020-10-26 11:50:03